Hooked on Biology

Today, I'm picking a topic solely because there was a funny mug I saw that requires this entire background on Sanger Sequencing. I'll send the picture too. Overview: - What it is/what it's used for - How it works - Data processing - History This one is going to be long but for anyone who's going into anything remotely related to genetics, it's good to know. Also, you will not fully understand the joke without the first three sections. What it is + What it's Used for Sanger Sequencing is a DNA sequencing technique developed by Frederick Sanger & co in 1977. It uses electrophoresis  which is a technique that takes advantage of DNA's negative charge to run it through a gel using a current. It's pretty accurate (99.99% according to Wikipedia) and can sequence a good number of nucleotides so it's still commonly in use, despite the invention of next generation (next gen) sequencing. How it Works: Dye-Terminator Sequencing  Sequencing starts with a proc...